THE EFFECT OF TOXOPLASMAS ON THE CHANGES IN THE EXPRESSION OF BIRC5, ERBB-2/HER2-NEU, GLI, VEGF PROTOONCOGENES AND THE ANTI-ONCOGENE TP53 IN RATS IN THE EXPERIMENT

Objectives. To study the effect of toxoplasmas on changes in expression protooncogenes BIRC5, ERBB-2/HER2-NEU, GLI, VEGF and anti-oncogene TP53 rats an experiment depending infection dose period parasite development. Material methods.The was conducted female Wistar to determine survivin (BIRC5), epidermal growth factor (ErbB-2/HER2-Neu), GLI 1, vascular endothelial (VEGF) comparison with reference genes β-actin (ACTB) GAPDH by means PCR analysis tissues 10 healthy 120 animals invaded at different doses. Statistical results all groups drawn data «control» series (healthy animals, biopsies lungs, liver, spleen, brain). The obtained experimental were as follows: 25 toxoplasma tachyzoites per 1 g animal body weight (5000 female) 50 (10000 female), then they also compared each other. processing carried out using program Statistica 10.0. differences considered be reliable a significance level less than 0.05 (p<0.05). Results. Toxoplasma found cause dose-dependent increase change strength stages Conclusions. Experimental toxoplasmosis alters (VEGF), intermediate host.